huh7 cell line Search Results


huh7  (Korean Cell Line Bank)
86
Korean Cell Line Bank huh7
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Huh7, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pmc08158706-106-16-17?v=Korean+Cell+Line+Bank
Average 86 stars, based on 1 article reviews
huh7 - by Bioz Stars, 2026-07
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huh7 cells  (LGC Promochem)
90
LGC Promochem huh7 cells
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Huh7 Cells, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pm17347668-176-0-14?v=LGC+Promochem
Average 90 stars, based on 1 article reviews
huh7 cells - by Bioz Stars, 2026-07
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human hepatoma cell line huh-7 bearing the hcv genotype 1b replicon  (Idenix Inc)
90
Idenix Inc human hepatoma cell line huh-7 bearing the hcv genotype 1b replicon
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Human Hepatoma Cell Line Huh 7 Bearing The Hcv Genotype 1b Replicon, supplied by Idenix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pm26479028-454-8-26?v=Idenix+Inc
Average 90 stars, based on 1 article reviews
human hepatoma cell line huh-7 bearing the hcv genotype 1b replicon - by Bioz Stars, 2026-07
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human hepatocyte-derived cellular carcinoma huh7 cell line  (Marburg GmbH)
90
Marburg GmbH human hepatocyte-derived cellular carcinoma huh7 cell line
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Human Hepatocyte Derived Cellular Carcinoma Huh7 Cell Line, supplied by Marburg GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pmc08129603-263-1-11?v=Marburg+GmbH
Average 90 stars, based on 1 article reviews
human hepatocyte-derived cellular carcinoma huh7 cell line - by Bioz Stars, 2026-07
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human hepatoma cancer cell line huh7  (National Centre for Cell Science)
90
National Centre for Cell Science human hepatoma cancer cell line huh7
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Human Hepatoma Cancer Cell Line Huh7, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pmc08536100-80-8-16?v=National+Centre+for+Cell+Science
Average 90 stars, based on 1 article reviews
human hepatoma cancer cell line huh7 - by Bioz Stars, 2026-07
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huh7 cell line  (Toni Lindl GmbH)
90
Toni Lindl GmbH huh7 cell line
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Huh7 Cell Line, supplied by Toni Lindl GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pmc06345847-241-23-27?v=Toni+Lindl+GmbH
Average 90 stars, based on 1 article reviews
huh7 cell line - by Bioz Stars, 2026-07
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huh7 cell line  (CEM Corporation)
90
CEM Corporation huh7 cell line
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Huh7 Cell Line, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/10__1016_slash_j__bmcl__2018__05__012-86-230-21?v=CEM+Corporation
Average 90 stars, based on 1 article reviews
huh7 cell line - by Bioz Stars, 2026-07
90/100 stars
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gene juice  (Merck KGaA)
90
Merck KGaA gene juice
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Gene Juice, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pmc03584027-44-6-28?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
gene juice - by Bioz Stars, 2026-07
90/100 stars
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huh7 cell line  (Microsynth ag)
90
Microsynth ag huh7 cell line
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Huh7 Cell Line, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pmc11156872-290-18-16?v=Microsynth+ag
Average 90 stars, based on 1 article reviews
huh7 cell line - by Bioz Stars, 2026-07
90/100 stars
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stable cell line huh7 cells  (GLACO LTD)
90
GLACO LTD stable cell line huh7 cells
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Stable Cell Line Huh7 Cells, supplied by GLACO LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pm36631545-155-6-18?v=GLACO+LTD
Average 90 stars, based on 1 article reviews
stable cell line huh7 cells - by Bioz Stars, 2026-07
90/100 stars
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human hepatoma cell line huh7  (Dr Raymond Laboratories Inc)
90
Dr Raymond Laboratories Inc human hepatoma cell line huh7
FGL1 expression level and viability in HepG2, <t>Huh7,</t> Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).
Human Hepatoma Cell Line Huh7, supplied by Dr Raymond Laboratories Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/10__1074_slash_jbc__m115__689190-69-2-11?v=Dr+Raymond+Laboratories+Inc
Average 90 stars, based on 1 article reviews
human hepatoma cell line huh7 - by Bioz Stars, 2026-07
90/100 stars
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huh7 cell line  (ScienCell)
90
ScienCell huh7 cell line
Functional experiments were performed in HCC cell lines with knockout and overexpression of INTS13. A, B CCK-8 cell proliferation assay results in Hep3B and <t>Huh7</t> cells as the indicated treatment. C–F Cell proliferation of Hep3B and Huh7 cells measured by EdU assays and quantification of EdU results after indicated treatment. G, H The expression of apoptosis-related proteins was analyzed in Hep3B and Huh7 cells analyzed by western blotting.
Huh7 Cell Line, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh7+cell+line/pmc11076979-68-6-10?v=ScienCell
Average 90 stars, based on 1 article reviews
huh7 cell line - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


FGL1 expression level and viability in HepG2, Huh7, Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: FGL1 expression level and viability in HepG2, Huh7, Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Expressing, Western Blot, Standard Deviation

IC 50 of sorafenib against HCC cell lines.

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: IC 50 of sorafenib against HCC cell lines.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques:

Effect of sorafenib on the cell colony forming capacity and cell death of HepG2, Huh7, Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Number of colonies after seeding 200 cells of each HCC cell line in the plate. ( B ) Effect of sorafenib on the colony forming potential of HCC cells. The six HCC cell lines were incubated for 7 days in the presence of 0, 2, or 5 µM sorafenib. Data are presented as the mean ± standard deviation (SD) (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from 0 µM sorafenib-treated cells. ( C ): Effect of sorafenib on cell death measured by flow cytometry using Annexin V-FITC and propidium iodide (PI) staining. The graph presents mean ± SD values of three independent experiments.

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of sorafenib on the cell colony forming capacity and cell death of HepG2, Huh7, Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Number of colonies after seeding 200 cells of each HCC cell line in the plate. ( B ) Effect of sorafenib on the colony forming potential of HCC cells. The six HCC cell lines were incubated for 7 days in the presence of 0, 2, or 5 µM sorafenib. Data are presented as the mean ± standard deviation (SD) (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from 0 µM sorafenib-treated cells. ( C ): Effect of sorafenib on cell death measured by flow cytometry using Annexin V-FITC and propidium iodide (PI) staining. The graph presents mean ± SD values of three independent experiments.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Incubation, Standard Deviation, Flow Cytometry, Staining

Effect of sorafenib on cell death and cell proliferation factors in high FGL1- and low FGL1-expressing hepatocellular carcinoma (HCC) cell lines. ( A ): Protein expression was analyzed by western blotting 48 h after treatment with 10 μM sorafenib. Expression levels of PCNA, p-ERK/ERK, LC3-II, and cleaved PARP1 quantified in Huh7 ( B ), Hep3B ( C ), SNU387 ( D ), and SNU475 ( E ) cell lines. Data are presented as the mean ± standard deviation (SD) (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from 0 µM sorafenib-treated cells.

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of sorafenib on cell death and cell proliferation factors in high FGL1- and low FGL1-expressing hepatocellular carcinoma (HCC) cell lines. ( A ): Protein expression was analyzed by western blotting 48 h after treatment with 10 μM sorafenib. Expression levels of PCNA, p-ERK/ERK, LC3-II, and cleaved PARP1 quantified in Huh7 ( B ), Hep3B ( C ), SNU387 ( D ), and SNU475 ( E ) cell lines. Data are presented as the mean ± standard deviation (SD) (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from 0 µM sorafenib-treated cells.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Expressing, Western Blot, Standard Deviation

Effect of silencing FGL1 in Huh7 and Hep3B cells on their sorafenib sensitivity. ( A ) Expression of FGL1 in Huh7 cells 24 h after FGL1 siRNA transfection. ( B ) Colony formation of Huh7 cells following siFGL1 and/or sorafenib (2 µM) treatment. ( C ) Expression of FGL1 in Hep3B cells 24 h after FGL1 siRNA transfection. ( D ) Colony formation of Hep3B cells following siFGL1 and/or sorafenib (2 µM) treatment. Graphs present mean ± standard deviation values (n = 4). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from scrambled siRNA-treated cells. siFGL1 (−), treated with siScr; Sorafenib (−), treated with vehicle (DMSO); siScr, scrambled siRNA; siFGL1, FGL1 siRNA.

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of silencing FGL1 in Huh7 and Hep3B cells on their sorafenib sensitivity. ( A ) Expression of FGL1 in Huh7 cells 24 h after FGL1 siRNA transfection. ( B ) Colony formation of Huh7 cells following siFGL1 and/or sorafenib (2 µM) treatment. ( C ) Expression of FGL1 in Hep3B cells 24 h after FGL1 siRNA transfection. ( D ) Colony formation of Hep3B cells following siFGL1 and/or sorafenib (2 µM) treatment. Graphs present mean ± standard deviation values (n = 4). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from scrambled siRNA-treated cells. siFGL1 (−), treated with siScr; Sorafenib (−), treated with vehicle (DMSO); siScr, scrambled siRNA; siFGL1, FGL1 siRNA.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Expressing, Transfection, Standard Deviation

Effect of FGL1 knockdown on sorafenib-induced cell death in Huh7 and Hep3B cell lines. Flow cytometry results of Huh7 ( A , B ) and Hep3B ( C , D ) cells are shown. The cell death percentage was estimated using the sum of the upper left, upper right, and lower right quadrants in each dot plot. The graphs present percentage of cell death. All values are presented as the mean ± standard deviation of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from scrambled siRNA-treated cells; # p < 0.05, significantly different from FGL1 siRNA-treated cells. siFGL1 (−), treated with siScr; Sorafenib (−), treated with vehicle (DMSO); siScr, scrambled siRNA; siFGL1, FGL1 siRNA.

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of FGL1 knockdown on sorafenib-induced cell death in Huh7 and Hep3B cell lines. Flow cytometry results of Huh7 ( A , B ) and Hep3B ( C , D ) cells are shown. The cell death percentage was estimated using the sum of the upper left, upper right, and lower right quadrants in each dot plot. The graphs present percentage of cell death. All values are presented as the mean ± standard deviation of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from scrambled siRNA-treated cells; # p < 0.05, significantly different from FGL1 siRNA-treated cells. siFGL1 (−), treated with siScr; Sorafenib (−), treated with vehicle (DMSO); siScr, scrambled siRNA; siFGL1, FGL1 siRNA.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Knockdown, Flow Cytometry, Standard Deviation

Effect of FGL1 knockdown on p-ERK, ERK, Beclin-1, and LC3-II in hepatocellular carcinoma (HCC) cells following sorafenib treatment. Representative western blot and data from Huh7 ( A ) and Hep3B ( B ) cells. Data are presented as the mean ± standard deviation (n = 3). * p < 0.05 and ** p < 0.01, compared to siScr-treated cells. siFGL1 (−), treated with siScr. Sorafenib (−), treated with vehicle (DMSO).

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of FGL1 knockdown on p-ERK, ERK, Beclin-1, and LC3-II in hepatocellular carcinoma (HCC) cells following sorafenib treatment. Representative western blot and data from Huh7 ( A ) and Hep3B ( B ) cells. Data are presented as the mean ± standard deviation (n = 3). * p < 0.05 and ** p < 0.01, compared to siScr-treated cells. siFGL1 (−), treated with siScr. Sorafenib (−), treated with vehicle (DMSO).

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Knockdown, Western Blot, Standard Deviation

Functional experiments were performed in HCC cell lines with knockout and overexpression of INTS13. A, B CCK-8 cell proliferation assay results in Hep3B and Huh7 cells as the indicated treatment. C–F Cell proliferation of Hep3B and Huh7 cells measured by EdU assays and quantification of EdU results after indicated treatment. G, H The expression of apoptosis-related proteins was analyzed in Hep3B and Huh7 cells analyzed by western blotting.

Journal: Heliyon

Article Title: Comprehensive analysis of INTS family related to expression, prognosis, diagnosis and immune features in hepatocellular carcinoma

doi: 10.1016/j.heliyon.2024.e30244

Figure Lengend Snippet: Functional experiments were performed in HCC cell lines with knockout and overexpression of INTS13. A, B CCK-8 cell proliferation assay results in Hep3B and Huh7 cells as the indicated treatment. C–F Cell proliferation of Hep3B and Huh7 cells measured by EdU assays and quantification of EdU results after indicated treatment. G, H The expression of apoptosis-related proteins was analyzed in Hep3B and Huh7 cells analyzed by western blotting.

Article Snippet: Human HCC cell lines, Hep3B and Huh7, were acquired from ScienCell Research Laboratories and cultured in DMEM supplemented with 10 % fetal bovine serum (Gibco, Carlsbad, CA, USA).

Techniques: Functional Assay, Knock-Out, Over Expression, CCK-8 Assay, Proliferation Assay, Expressing, Western Blot